rig1 wb cell signaling technologies  (Cell Signaling Technology Inc)

Journal: Blood Cancer Journal

Article Title: Early growth response 1 as a key regulator of PD-L1 expression and immune evasion in extranodal NK/T-cell lymphoma

doi: 10.1038/s41408-025-01313-w

Figure Lengend Snippet: A Baseline expression of LMP1, RIG1, EGR1, and PD-L1 in ENKTL cell lines. B Effect of EGR1 knockdown on LMP1 and PD-L1 expression. C Flow cytometric analysis of surface PD-L1 expression following EGR1 knockdown. D EGR1 overexpression-driven enhancement of LMP1 and PD-L1 in SNK6 Cells.

Article Snippet: RIG1 (sc-376845) and GAPDH (sc25778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Expressing, Knockdown, Over Expression

Journal: Archives of Virology

Article Title: An NLR family member X1 mutation ( p.Arg707Cys ) suppresses hepatitis B virus infection in hepatocytes and favors the interaction of retinoic acid-inducible gene 1 with mitochondrial antiviral signaling protein

doi: 10.1007/s00705-024-06133-0

Figure Lengend Snippet: Effect of the NLRX1 mutation on MAVS/RIG1 and proinflammatory cytokines. Huh7-NTCP cells are transfected with WT NLRX1 or MT NLRX1 vector for 48 h. ( A ) Coimmunoprecipitation assay showing the effect of WT NLRX1 or MT NLRX1 on MAVS/RIG1 interaction. ( B ) Luciferase expression from the IFN-α, IL-6, or IFN-β promoter in Huh7-NTCP cells. All experiments were repeated three times independently. *, P < 0.05 vs. NC + HBV; **, P < 0.01 vs. NC + HBV; +, P < 0.05 vs. WT NLRX1 + HBV

Article Snippet: The primary antibodies were as follows: HBcAg (Santa Cruz, #sc-23947; 1:1000); HBsAg (Santa Cruz, #sc-53299; 1:1000); NLRX1 (Proteintech, Wuhan, China, #17215-1-AP; 1:2000); RIG1 (CST, #3743T; 1:2000); MAVS (Abcam, ab31334; 1:2000); p65 (Abcam, ab7970; 1:500); p-p65 (CST, #3033S; 1:1000); IRF7 (CST, #13014; 1:1000); p-IRF7 (CST, #12390; 1:1000); IRF3 (CST, #4302; 1:1000); p-IRF3 (CST, #29047; 1:1000); and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Proteintech, 60004-1-Ig, 1:8000).

Techniques: Mutagenesis, Transfection, Plasmid Preparation, Co-Immunoprecipitation Assay, Luciferase, Expressing